Show simple item record

dc.contributor.authorWithers, John
dc.contributor.authorYao, Jian
dc.contributor.authorMecey, Christy
dc.contributor.authorHowe, Gregg A.
dc.contributor.authorMelotto, Maeli
dc.contributor.authorHe, Sheng Yang
dc.date.accessioned2019-10-24T23:10:30Z
dc.date.available2019-10-24T23:10:30Z
dc.date.created2017-02-28 02:10
dc.date.issued2012-12-04
dc.identifieroai:pubmedcentral.nih.gov:3523844
dc.identifier/pmc/articles/PMC3523844/
dc.identifier/pubmed/23169619
dc.identifierhttp://dx.doi.org/10.1073/pnas.1210054109
dc.identifier.urihttp://hdl.handle.net/20.500.12424/1151533
dc.description.abstractThe plant hormone jasmonate (JA) plays an important role in regulating growth, development and immunity. A key step in JA signaling is ligand-dependent assembly of a coreceptor complex consisting of the F-box protein COI1 and JAZ transcriptional repressors. Assembly of this receptor complex results in proteasome-mediated degradation of JAZ repressors, which at resting state bind to and repress the MYC transcription factors. Although the JA receptor complex is believed to function within the nucleus, how this receptor complex enters the nucleus and, more generally, the cell biology of jasmonate signaling are not well understood. In this study, we conducted mutational analysis of the C termini (containing the conserved Jas motif) of two JAZ repressors, JAZ1 and JAZ9. These analyses unexpectedly revealed different subcellular localization patterns of JAZ1ΔJas and JAZ9ΔJas, which were associated with differential interaction of JAZ1ΔJas and JAZ9ΔJas with MYC2 and differential repressor activity in vivo. Importantly, physical interaction with MYC2 appears to play an active role in the nuclear targeting of JAZ1 and JAZ9, and the nuclear localization of JAZ9 was compromised in myc2 mutant plants. We identified a highly conserved arginine residue in the Jas motif that is critical for coupling MYC2 interaction with nuclear localization of JAZ9 and JAZ9 repressor function in vivo. Our results suggest a model for explaining why some JAZΔJas proteins, but not others, confer constitutive JA-insensitivity when overexpressed in plants. Results also provide evidence for a transcription factor-dependent mechanism for nuclear import of a cognate transcriptional repressor JAZ9 in plants.
dc.languageen
dc.language.isoeng
dc.publisherNational Academy of Sciences
dc.subjectBiological Sciences
dc.titleTranscription factor-dependent nuclear localization of a transcriptional repressor in jasmonate hormone signaling
dc.typeText
ge.collectioncodeOAIDATA
ge.dataimportlabelOAI metadata object
ge.identifier.legacyglobethics:10814714
ge.identifier.permalinkhttps://www.globethics.net/gtl/10814714
ge.lastmodificationdate2017-02-28 02:10
ge.lastmodificationuseradmin@pointsoftware.ch (import)
ge.submissions0
ge.oai.exportid149001
ge.oai.repositoryid1570
ge.oai.setnameProceedings of the National Academy of Sciences of the United States of America
ge.oai.setspecpnas
ge.oai.streamid5
ge.setnameGlobeTheoLib
ge.setspecglobetheolib
ge.linkhttps://dx.doi.org/10.1073/pnas.1210054109


This item appears in the following Collection(s)

Show simple item record