Effect of Zinc Sulfate and Ascorbic Acid on some Morpho-Physiological Traits of Echinacea purpurea (Purple coneflower) under Water Deficit Conditions
KeywordsEchinaceae purpurea (L.) Monch
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AbstractTo quantify the response of some morpho-physiological traits of Echinacea purpurea to thr application of zinc sulfate and ascorbic acid under water deficit, an experiment was carried out in split plot factorial based on RCBD with three replications at the Agricultural Research Station of Islamic Azad University, Tabriz Branch in 2011-12. Experimental factors consisted of water stress as the main factor with three levels (irrigation after70mm evaporation, irrigation after120mm evaporation and irrigation after 170mm evaporation from class A pan), Secondary factor consisted of: application of microelement with two levels (control and application of zinc sulfate 0.005 concentration) and ascorbic acid with four levels (not application, application of 50 mg/l of ascorbic acid, application of 100 mg/l of ascorbic acid and application of 150 mg/l of ascorbic acid). The results showed that the effect of water deficit on purple coneflower caused significant differences in diameter of stem, number of flowering branch, stomata density, zinc concentration of aerial parts plant, fresh weight, relative water content of leaf, LAI, proline concentration, crop growth rate, relative growth rate and net assimilation rate. Comparison of means for water deficits between the different levels of drought stress showed that the maximum LAI, with 2.85, belonged to control. Comparison of means for interaction effects between drought stress and application of zinc sulfate revealed that the maximum proline concentration belonged to spraying plants with zinc sulfate at irrigation after 170mm evaporation from class A pan with 10.16 mg/g.fw. Minimum proline concentration was due to without applying zinc sulfate in complete irrigation (control) with 0.08 mg/g.fw. Maximum crop growth rate with 6.77 g/m2.day was was related to control and the minimum, with 4.16 g/m2.day, to irrigation after 170mm. Maximum relative crop growth rate, with 0.19 g/m2.day, belonged to control and the minimum, with 0.14 g/m2.day, to irrigation after 170mm. Maximum net assimilation rate, with 2.64 g/m2.day, belonged to control and the minimum, with 1.91 g/m2.day, to irrigation after 170mm. The results indicated that application of ascorbic acid, as an antioxidant, may decrease the harmful effects of drought stress on some traits (NAR, CGR, RGR, and RWC) of purple coneflower.