AbstractA field experiment was conducted for two successive seasons 2012/2013 and 2013/2014 with four replicates at the experimental farm, Faculty of Agriculture, Omdurman Islamic University to determine the effect of inoculation and co-inoculation of symbiotic nitrogen fixing and phosphate solubilizing bacteria on fenugreek (Trigonella foenum-graecum L.) growth and yield. Fenugreek plant variety Kodab was used. Two bacterial strains Sinorhizobium meliloti strain TAL380 and Bacillus megatherium var. phosphaticum strain (BMP), each alone or in combination. Control plants were kept for comparison. The following measurements were taken per plant: number of nodules, dry weight of nodules, shoots and roots, plant height, pod length, number of pods/plant, number of seeds/pod, 1000 seed weight and yield. Results showed that inoculation with TAL380 and BMP each alone significantly increased nodulation and nodule dry weight compared to the control. Inoculation with BMP significantly increased root dry weight, when inoculation with TAL380 significantly increased shoot dry weight and plant height, co-inoculation significantly increased root and shoot dry weight compared to the control. The two bacterial inoculations each alone or in combination significantly increased number of pods and insignificantly increased number of seeds per pod and 1000 seed weight at both seasons. There was no effect of inoculation with bacteria on pod length. Co-inoculation significantly increased fenugreek seed yield at both seasons compared to the control.