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dc.contributorThe Pennsylvania State University CiteSeerX Archives
dc.contributor.authorDevon M. Fitzgerald
dc.contributor.authorMichael E. Charness
dc.contributor.authorKimberly A. Leite-morris
dc.contributor.authorSuzhen Chen
dc.date.accessioned2019-10-28T22:28:14Z
dc.date.available2019-10-28T22:28:14Z
dc.date.created2018-09-05 00:54
dc.date.issued2013-07-10
dc.identifieroai:CiteSeerX.psu:10.1.1.290.1384
dc.identifierhttp://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.1384
dc.identifier.urihttp://hdl.handle.net/20.500.12424/2514337
dc.description.abstractThe neural cell adhesion molecule L1 is critical for brain development and plays a role in learning and memory in the adult. Ethanol inhibits L1-mediated cell adhesion and neurite outgrowth in cerebellar granule neurons (CGNs), and these actions might underlie the cerebellar dysmorphology of fetal alcohol spectrum disorders. The peptide NAP potently blocks ethanol inhibition of L1 adhesion and prevents ethanol teratogenesis. We used quantitative RT-PCR and Western blotting of extracts of cerebellar slices, CGNs, and astrocytes from postnatal day 7 (PD7) rats to investigate whether ethanol and NAP act in part by regulating the expression of L1. Treatment of cerebellar slices with 20 mM ethanol, 10 212 M NAP, or both for 4 hours, 24 hours, and 10 days did not significantly affect L1 mRNA and protein levels. Similar treatment for 4 or 24 hours did not regulate L1 expression in primary cultures of CGNs and astrocytes, the predominant cerebellar cell types. Because ethanol also damages the adult cerebellum, we studied the effects of chronic ethanol exposure in adult rats. One year of binge drinking did not alter L1 gene and protein expression in extracts from whole cerebellum. Thus, ethanol does not alter L1 expression in the developing or adult cerebellum; more likely, ethanol disrupts L1 function by modifying its conformation
dc.format.mediumapplication/zip
dc.languageen
dc.language.isoeng
dc.rightsMetadata may be used without restrictions as long as the oai identifier remains attached to it.
dc.titleEffects of Ethanol and NAP on Cerebellar Expression of the Neural Cell Adhesion Molecule L1
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ge.dataimportlabelOAI metadata object
ge.identifier.legacyglobethics:15165075
ge.identifier.permalinkhttps://www.globethics.net/gel/15165075
ge.lastmodificationdate2018-09-05 00:54
ge.lastmodificationuseradmin@pointsoftware.ch (import)
ge.submissions0
ge.oai.exportid149801
ge.oai.repositoryid54
ge.oai.streamid2
ge.setnameGlobeEthicsLib
ge.setspecglobeethicslib
ge.linkhttp://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.1384
ge.linkftp://ftp.ncbi.nlm.nih.gov/pub/pmc/ec/ff/PLoS_One_2011_Sep_8_6(9)_e24364.tar.gz


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