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dc.contributor.authorHoltmaat, A.
dc.contributor.authorDe Paola, V.
dc.contributor.authorWilbrecht, L.
dc.contributor.authorKnott, G. W.
dc.date.accessioned2019-10-29T00:47:36Z
dc.date.available2019-10-29T00:47:36Z
dc.date.created2018-09-05 01:04
dc.date.issued2008-09-01
dc.identifieroai:repository.cshl.edu:27548
dc.identifierHoltmaat, A., De Paola, V., Wilbrecht, L., Knott, G. W. (2008) Imaging of experience-dependent structural plasticity in the mouse neocortex in vivo. Behav Brain Res, 192 (1). pp. 20-25.
dc.identifier.urihttp://hdl.handle.net/20.500.12424/2542376
dc.description.abstractThe functionality of adult neocortical circuits can be altered by novel experiences or learning. This functional plasticity appears to rely on changes in the strength of neuronal connections that were established during development. Here we will describe some of our studies in which we have addressed whether structural changes, including the remodeling of axons and dendrites with synapse formation and elimination, could underlie experience-dependent plasticity in the adult neocortex. Using 2-photon laser-scanning microscopes and transgenic mice expressing GFP in a subset of pyramidal cells, we have observed that a small subset of dendritic spines continuously appear and disappear on a daily basis, whereas the majority of spines persists for months. Axonal boutons from different neuronal classes displayed similar behavior, although the extent of remodeling varied. Under baseline conditions, new spines in the barrel cortex were mostly transient and rarely survived for more than a week. However, when every other whisker was trimmed, the generation and loss of persistent spines was enhanced. Ultrastructural reconstruction of previously imaged spines and boutons showed that new spines slowly form synapses. New spines persisting for a few days always had synapses, whereas very young spines often lacked synapses. New synapses were predominantly found on large, multi-synapse boutons, suggesting that spine growth is followed by synapse formation, preferentially on existing boutons. Altogether our data indicate that novel sensory experience drives the stabilization of new spines on subclasses of cortical neurons and promotes the formation of new synapses. These synaptic changes likely underlie experience-dependent functional remodeling of specific neocortical circuits.
dc.relation.ispartofhttp://www.ncbi.nlm.nih.gov/pubmed/18501438
dc.relation.ispartofhttp://repository.cshl.edu/27548/
dc.subjectInvestigative techniques and equipment
dc.subjectanimal
dc.subjectbrain
dc.subjectcell functions
dc.subjectcell types and functions
dc.subjectimaging
dc.subjectmouse
dc.subjectneural plasticity
dc.subjectorgans types and functions
dc.subjectorgans, tissues, organelles, cell types and functions
dc.titleImaging of experience-dependent structural plasticity in the mouse neocortex in vivo
dc.typePaper
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ge.identifier.legacyglobethics:15194189
ge.identifier.permalinkhttps://www.globethics.net/gel/15194189
ge.lastmodificationdate2018-09-05 01:04
ge.lastmodificationuseradmin@pointsoftware.ch (import)
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ge.oai.setnameStatus = Published
ge.oai.setnameSubject = Investigative techniques and equipment
ge.oai.setnameSubject = organism description: animal
ge.oai.setnameSubject = organs, tissues, organelles, cell types and functions: organs types and functions: brain
ge.oai.setnameSubject = organs, tissues, organelles, cell types and functions: cell types and functions: cell functions
ge.oai.setnameSubject = organs, tissues, organelles, cell types and functions: cell types and functions
ge.oai.setnameSubject = Investigative techniques and equipment: imaging
ge.oai.setnameSubject = organism description: animal: mammal: rodent: mouse
ge.oai.setnameSubject = organs, tissues, organelles, cell types and functions: cell types and functions: cell functions: neural plasticity
ge.oai.setnameSubject = organs, tissues, organelles, cell types and functions: organs types and functions
ge.oai.setnameSubject = organs, tissues, organelles, cell types and functions
ge.oai.setnameType = Paper
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ge.linkhttp://www.ncbi.nlm.nih.gov/pubmed/18501438
ge.linkhttp://repository.cshl.edu/27548/


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