Development of Effective Aerobic Cometabolic Systems for the In Situ Transformation of Problematic Chlorinated Solvent Mixtures
Water Pollution and Control
*WATER POLLUTION ABATEMENT
TEST AND EVALUATION
PASSIVE IN-SITU TREATMENT
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AbstractMany sites in the DoD and DOE complex are contaminated with chlorinated solvent mixtures. Passive in-situ treatment via aerobic cometabolism is one means of potentially restoring the contaminated aquifers. The goal of the project was to develop a cometabolic culture that has potential for bioaugmentation and to evaluate its performance under laboratory conditions and under in-situ conditions in field demonstrations. The specific objectives of this study were to: 1) develop a butane utilizing culture for bioaugmentation in laboratory and field experiments; 2) characterize the bioaugmentation culture using molecular methods, including a clone library, sequencing, and PCR based methods; 3) develop kinetic information for substrate utilization and the transformation of the CAH mixtures; 4) develop molecular based methods for tracking the cultures that were bioaugmented and biostimulated in laboratory and field studies; 5) conduct laboratory microcosm and continuous flow column studies to evaluate the performance of the bioaugmented culture under geochemical conditions mimicking those present at the field site; 6) conduct field demonstrations to evaluate the bioaugmentation approach and to determine the effectiveness in treating problematic mixtures of 1,1,1-TCA, 1,1-DCE, and 1,1-DCA using butane as cometabolic substrate, and compare the results to those achieved by indigenous butane-utilizers; 7) track microbial community changes and quantify members of the bioaugmented culture in situ using molecular based methods; 8) simulate the results of laboratory and field studies using a transport code for these cometabolic transformations.
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